This article, first takes a formalist approach and attempts to achieve two goals: defining the term defamiliarization, its essence and function. Then, it illustrates one of the prosodic features of Naser khosrow’s poetry which could be considered as an appropriate example of rhythmic defamiliarization. For this purpose, seven meters from his Divān, of which rare meters are usually called unpleasant/ unfamiliar, will be discussed; and, according to their different structures, they will be categorized into  two groups: one incorporating six meters, and the other containing only one. The meters of the first group which are mainly analyzed in this study have different length from their current forms. When a poet such as Naser Khosrow employs these infrequent meters in his poetry, it sounds odd to most of Persian readers who are accustomed to the ordinary rhythms. This can be called rhythmic/ prosodic defamiliarization.